Figure 5.

Chromosomal instability in UHRF1-depleted cells. (A-D) Representative photomicrographs showing presence of micronuclei in UHRF1-depleted cells. (E) Percentage of cells with micronuclei in control cells vs. UHRF1-depleted cells. Determinations from each cell line were performed in triplicate (n ≥ 200 cells/determination) and error bars represent standard deviations from the mean. (F) Percentage of control or UHRF1-depleted cells with 1, 2, 3, or 4 centrosomes per cell. (G-I) Representative images of DAPI-stained metaphases from UHRF1-depleted cells showing chromosomal aberrations (image shown in H is magnification of image in G). br = chromosome break, cf = centrosomal fragment, af = acentric fragments. Telomeres are visualized in red, centromeres in green.